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. 2019 Feb 28;10:147. doi: 10.3389/fphar.2019.00147

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Copyright © 2019 Ghareghani, Scavo, Jand, Farhadi, Sadeghi, Ghanbari, Mondello, Arnoult, Gharaghani and Zibara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of melatonin on PDK4 levels in brain homogenate. (A) mRNA expression levels of PDK4 assessed by real time-PCR. (B) Protein expression levels of PDK4 assessed by western-blot. β-actin was used as an internal control for normalization for both real time-PCR and western blot. Quantification PDK4 expression levels was normalized to controls. Values are expressed as the Mean ± SEM. Each group included 8 mice (n = 8). Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by Tukey’s test. Significance is indicated by ^∗∗∗p < 0.001 and ^∗∗∗∗p < 0.001 vs. control-PBS and ^###p < 0.001 vs. EAE-PBS.