Table 1.
Characteristics of isolates obtained from Lake Água Preta.
| Isolate | 16S rRNA gene Affiliation | Resistance (and intermediate) phenotype | Resistance genotype | Integrase genes |
|---|---|---|---|---|
| APC43A | Escherichia coli NBRC 102203 (99%) | AML, AMC, AMP, CEF, CAZ, CTX, FEP, ATM, CIP, NAL (KAN)b | blaCTX–M–15a | – |
| APC25 | Acinetobacter baumannii DSM 30007 (97%) | AML, AMC, AMP, CEF, CAZ, CTX, ATM, CIP, NAL (KAN) | –a | – |
| APC4 | Citrobacter werkmanii CDC 0876-58 (99%) | AML, AMC, AMP, CEF, CAZ, CTX, ATM, CIP, NAL, SXT, TET (KAN) | – | – |
| APC6 | Enterobacter sp. A2 (99%) | AML, AMC, AMP, CEF, CAZ, ATM, CTX, CIP, GEN, KAN, NAL, TET | blaTEM-blaSHV | – |
| APC11 | Pseudomonas putida F1 (99%) | AML, AMC, AMP, CEF, CAZ, CTX, ATM, CHL, NAL, SXT | blaCTX–M | – |
| APC13 | Acinetobacter baumannii DSM 30007 (97%) | AML, AMP, CEF, CAZ, FEP, ATM (CTX, NAL) | – | – |
| APC14 | Pseudomonas mosselii CFML 90-83 (99%) | AML, AMC, AMP, CEF, CAZ, CTX, FEP, ATM, TET (NAL) | – | – |
| APC15 | Chromobacterium haemolyticum MDA0585 (99%) | AML, AMC, AMP, CEF, CAZ, CTX, FEP, IPM, ATM, KAN, NAL | – | – |
| APC19 | Escherichia coli O157:H7 Sakai (99%) | AML, AMC, AMP, CEF, CAZ, CTX, FEP, ATM, CIP, GEN, KAN, NAL, SXT, TET | blaCTX–M | intI2 |
| APC20 | Pseudomonas mosselii CFML 90-83 (99%) | AML, AMC, AMP, CEF, CAZ, CTX, FEP, ATM (NAL) | – | – |
| APC22 | Shigella sonnei Ss046 (99%) | AML, AMP, CEF, CTX, FEP, ATM, CIP, GEN, KAN, NAL, SXT (AMC) | blaCTX–M–2 | intI2 |
| APC24B | Escherichia fergusonii ATCC 35469 (99%) | AML, AMC, AMP, CEF, CAZ, CTX, FEP, ATM, CIP, KAN, NAL, SXT, TET | blaCTX–M | IntI1 |
| APC28 | Klebsiella pneumoniae DSM 30104 (96%) | AML, AMC, AMP, CEF, CAZ, CTX, FEP, ATM, CIP, KAN, NAL, SXT, TET (C) | blaTEM- blaSHV-blaCTX–M–15 | intI1 |
| APC32 | Escherichia fergusonii ATCC 35469 (97%) | AML, AMP, CEF, CAZ, CTX, FEP, ATM, SXT (AMC, CIP, KAN) | blaTEM-blaCTX–M–15 | – |
| APC33 | Shigella sonnei Ss046 (99%) | AML, AMC, AMP, CEF, CAZ, CTX, FEP, ATM, CIP, NAL | blaCTX–M–15 | – |
| APC34 | Escherichia coli NBRC 102203 (98%) | AML, AMC, AMP, CEF, CAZ, CTX, FEP, ATM, CIP, KAN, NAL, SXT, TET | blaCTX–M | IntI2 |
| APC38 | Escherichia fergusonii ATCC 35469 (98%) | AML, AMC, AMP, CEF, CTX, FEP, ATM, CIP, GEN, CHL, KAN, NAL, SXT, TET | blaCTX–M | IntI2 |
| APC39 | Acinetobacter nosocomialis RUH2376 (99%) | AML, AMC, AMP, CEF, CAZ, CTX, ATM, CIP, KAN, NAL, SXT, TET | – | – |
| APC40A | Escherichia fergusonii ATCC 35469 (99%) | AML, AMC, AMP, CEF, CAZ, CTX, FEP, ATM, CIP, GEN, CHL, NAL, SXT (KAN) | blaCTX–M–14 | intI1-intI2 |
| APC42 | Acinetobacter baumanii DSM 30007 (99%) | AML, AMC, AMP, CEF, CAZ, CTX, FEP, ATM, CIP, KAN, NAL, SXT, TET (CHL) | – | |
| APC43B | Shigella sonnei Ss046 (99%) | AML, AMC, AMP, CEF, CAZ, CTX, FEP, ATM, CIP, GEN, KAN, NAL, SXT, TET (CHL) | blaCTX–M–15 | – |
| API2 | Klebsiella pneumoniae R-70 (99%) | AML, ATM, C, KAN, SXT, TET (AMP, CIP) | – | IntI1 |
| API3 | Enterobacter asburiae JCM6051 (94%) | AML, AMC, AMP, CEF, CAZ, ATM, CHL, KAN, NAL, TET | – | – |
| API4 | Klebsiella pneumoniae 07A044 (99%) | AML, AMC, AMP, CEF, ATM, SXT, TET (CTX, NAL) | blaSHV | – |
| API6 | Leclercia adecarboxylata CIP 82.92 (99%) | AML, AMC, AMP, CEF, ATM, CHL, KAN, NAL, TET (CTX) | – | – |
| API7 | Enterobacter cloacae LMG 2683 (99%) | AML, AMC, AMP, CEF (CTX, ATM) | – | – |
| API10 | Enterobacter tabaci YIM Hb-3 (99%) | AML, AMC, AMP, CEF, ATM, CHL, KAN, NAL (SXT, TET) | – | – |
| API12 | Pseudomonas otitidis MCC10330 (99%) | AML, AMC, AMP, CEF, ATM, KAN, TET (NAL) | – | – |
| API16 | Enterobacter tabaci YIM Hb-3 (100%) | AML, AMC, AMP, CEF, ATM, GEN, CHL, NAL, SXT, TET (CTX) | – | – |
| API20 | Acinetobacter seifertii LUH 1472 (99%) | AML, AMC, AMP, CEF, ATM, CIP, KAN, TET (CAZ, CTX, GEN, CHL) | – | – |
| API24 | Phytobacter diazotrophicus Ls8 (98%) | AMP, CAZ, CTX, ATM, KAN, NAL (AML, FEP, TET) | blaTEM | – |
| API29 | Klebsiella pneumoniae DSM 30104 (99%) | AML, AMP, CEF | blaTEM- blaSHV | – |
| API34 | Klebsiella pneumoniae DSM 30104 (98%) | AML, AMP, CEF, CTX, FEP, ATM, CIP, GEN, CHL, NAL, SXT, TET | – | – |
The BLASTn identity result for each isolate is presented within parentheses after the 16S rRNA affiliation. The abbreviation of antibiotics is as follows: amoxicillin (AML); amoxicillin + clavulanic acid (AMC); ampicillin (AMP); cephalotin (CEF); ceftazidime (CAZ); cefotaxime (CTX); aztreonam (ATM); cefepime (FEP); imipenem (IPM); kanamycin (KAN); gentamicin (GEN); nalidixic acid (NAL); ciprofloxacin (CIP); chloramphenicol (CHL); tetracycline (TET); sulfamethoxazole + trimethoprim (SXT). aIsolates selected for whole genome analysis. The complete analysis of its resistance genotype is presented in main text and in Table 2. bParentheses indicate intermediate susceptibility to the antibiotic.