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. 2019 Feb 28;10:122. doi: 10.3389/fphys.2019.00122

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Copyright © 2019 Souza, Gama, Schama, Lima, Diaz-Albiter and Genta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activity against laminarin in fractions obtained after gel filtration chromatography (Superdex 200 – AKTA FPLC) of soluble fractions obtained from the (A) head, (B) digestive tract and (C) rest of the body of Ae. aegypti larvae. Elution volumes and molecular masses of protein standards were used to build a calibration curve for calculation of the molecular masses of β-1,3-glucanase activities. Elution volumes (mL)/fraction (number) of the standards were as follows: 18.22/34 (cytochrome C, 12.4 kDa), 16.86/32 (carbonic anhydrase, 29 kDa), 14.87/28 (bovine serum albumin, 66 kDa), 13.49/25 (alcohol dehydrogenase, 150 kDa), 12.77/24 (β-amylase, 200 kDa), and 5.64/9 (blue dextran, 2000 kDa). The elutions (mL/fraction number) of β-1,3-glucanase activities from (A) head, (B) gut, and (C) rest of body were 13.75/26, 16.75/32, and 13.5/25, respectively, resulting in predicted molecular masses of 142, 41, and 150 kDa.