FIGURE 4.

Relative expressions of the genes encoding GHF16 proteins in fourth instar larvae of Ae. aegypti, measured in series of semi-quantitative RT-PCR reactions with 24 or 27 cycles. Numbers are relative expressions normalized using the gene RP49 as a constitutive control. cDNA samples were prepared as described in “Materials and Methods” section from (A) whole L4 larvae (27 cycles), (B) heads (27 cycles), (C) guts (24 cycles) and (D) rest of bodies (27 cycles). 1–6 correspond to the relative expression levels of AaeGH16.1-6, respectively.