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. 2019 Feb 28;10:122. doi: 10.3389/fphys.2019.00122

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Copyright © 2019 Souza, Gama, Schama, Lima, Diaz-Albiter and Genta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ae. aegypti GHF16 genes have distinct physiological roles. (A) Survival curve of Ae. aegypti fourth instar larvae after feeding with dsRNA. dsGH1, dsGH4, dsGH5, dsGH6 correspond to larvae fed with dsRNA coding, respectively, for the genes AaeGH16.1, AaeGH16.4, AaeGH16.5, and AaeGH16.6. GFP and H₂O correspond to the control groups fed with dsGFP or water only. (B) Percentage of Ae. aegypti fourth instar larvae and pupae in after treatment with dsRNA and following for 12 days in control diet (cat food). GH1/GH4/GH5/GH6/GFP/H₂O correspond to larvae exposed to dsRNA coding for AaeGH16.1, AaeGH16.4, AaeGH16.5, AaeGH16.6, GFP, and water, respectively. Comparison of survival curves vs. the control treated with dsGFP used the Log-rank test (^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001) and comparison of groups vs. the control treated with dsGFP used the chi-square test (^∗p < 0.05; ^∗∗p < 0.01).