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. 2019 Feb 28;10:122. doi: 10.3389/fphys.2019.00122

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Copyright © 2019 Souza, Gama, Schama, Lima, Diaz-Albiter and Genta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Levels of expression of the genes AeGH16.4 (GH4), AeGH16.5 (GH5), and AeGH16.6 (GH6) in Ae. aegypti fourth instar larvae after treatment with dsRNA. (A) Relative expression of the gene AeGH16.4 in whole larvae. (B) Relative expression of the gene AeGH16.5 in the gut. (C) Relative expression of the gene AeGH16.6 in the rest of the body. Expression levels were normalized using the ribosomal RP gene 49 as the constitutive marker. We used 24 cycles for AeGH16.4 and 27 cycles for AeGH16.5 and AeGH16.6 in the RT-PCR reactions. See “Materials and Methods” for details.