FIGURE 5.

Effect of Raptor and Rictor shRNAs on the current density of endogenous BK channels in podocytes. (A) Representative traces of whole-cell currents from podocytes before and after application of 10 μM paxilline. The holding potential was -80 mV and the currents were evoked by step pulses ranging from -50 to +120 mV for 200 ms in increments of 10 mV and 5 μM free Ca²⁺ in the pipette solution. (B) Quantification of current densities. (C) Mean currents of cells transiently transfected with NT, Raptor, and Rictor shRNA. (D) Plots of the normalized conductance as a function of command potential in the absence of Raptor and Rictor. Data are expressed as mean ± SEM. ^∗P < 0.05 vs. NT shRNA. One-way ANOVA and Dunnett’s Multiple Comparison test (B,C).