Table 2.
Bacterial model mixtures for determining the efficiency of the Arcobacter-23S v-qPCR to detect DNA from live cells.
| Ct-value | 100% LC | ||||
|---|---|---|---|---|---|
| Species | OD550 = 0.250 | copy numbers | 50% LC a | 10% LC a | 100% DC a |
| A. aquimarinus | 14.23 ± 0.13 | 2.24 × 107 | 56.81 ± 22.45 | 15.43 ± 4.34 | 0.01 ± 0.01 |
| A. bivalviorum | 14.77 ± 0.18 | 1.41 × 107 | 66.04 ± 16.90 | 15.76 ± 5.26 | 0.01 ± 0.01 |
| A. butzleri | 13.33 ± 0.07 | 1.17 × 107 | 45.28 ± 5.54 | 11.85 ± 4.75 | 0.02 ± 0.01 |
| A. cibarius | 13.98 ± 0.32 | 3.11 × 107 | 59.11 ± 5.24 | 11.26 ± 1.56 | 0.04 ± 0.03 |
| A. cloacae | 13.46 ± 0.28 | 4.21 × 107 | 61.82 ± 11.73 | 14.58 ± 4.48 | ND |
| A. cryaerophilus | 12.54 ± 0.16 | 2.14 × 107 | 51.48 ± 4.54 | 8.39 ± 1.83 | 0.01 ± 0 |
| A. defluvii | 13.62 ± 0.21 | 3.96 × 107 | 40.08 ± 6.69 | 8.74 ± 1.01 | 0.24 ± 0.21 |
| A. ebronensis | 14.34 ± 0.21 | 1.88 × 107 | 38.56 ± 8.09 | 7.11 ± 1.06 | ND |
| A. ellisii | 12.83 ± 1.08 | 7.53 × 107 | 30.02∗ ± 4.53 | 8.16 ± 2.49 | 0.02 ± 0.01 |
| A. halophilus | 14.01 ± 0.14 | 2.10 × 107 | 67.42 ± 4.84 | 12.61 ± 1.17 | ND |
| A. lanthieri | 13.54 ± 0.12 | 4.97 × 107 | 47.85 ± 1.69 | 8.16 ± 0.62 | 0.01 ± 0 |
| A. marinus | 15.98 ± 0.13 | 5.62 × 106 | 71.43 ± 16.52 | 23.66 ± 1.96 | 0.5 ± 0.27 |
| A. molluscorum | 14.47 ± 0.46 | 1.80 × 107 | 24.99∗ ± 3.12 | 7.68 ± 6.09 | 0.12 ± 0.05 |
| A. mytili | 14.34 ± 0.03 | 2.57 × 107 | 56.98 ± 14.71 | 19.5 ± 15.6 | 0.63 ± 1.08 |
| A. nitrofigilis | 17.99 ± 0.21 | 3.52 × 106 | 58.06 ± 2.57 | 11.3 ± 0.96 | 0.03 ± 0.03 |
| A. skirrowii | 13.74 ± 0.21 | 1.12 × 107 | 50.32 ± 4.77 | 9.38 ± 1.85 | ND |
| A. suis | 14.78 ± 0.30 | 5.77 × 106 | 71.31∗ ± 4.2 | 19.09 ± 2.59 | 0.01 ± 0 |
| A. thereius | 15.54 ± 0.33 | 8.69 × 106 | 55.65 ± 13.2 | 9.04 ± 2.88 | ND |
| A. trophiarum | 14.94 ± 0.61 | 7.07 × 107 | 39.4 ± 23.57 | 6.96 ± 6.15 | 0.13 ± 0.21 |
| A. venerupis | 14.77 ± 0.22 | 2.06 × 107 | 30.28 ± 14.21 | 8.44 ± 4.88 | ND |
| Average | 14.36 ± 1.20 | 2.59 × 107 | 53.31 ± 21.97 | 12.32 ± 7.85 | 0.09 ± 0.27 |
aPercentage of copy numbers detected in relation to the total copy number obtained from the bacterial model of 100% live cells. ∗Mean percentage was statistically different from the rest (P = 0.05). LC, live cells; DC, dead cells; ND, no detection.