Table 3.
Quantitative detection of 23S copy numbers of Arcobacter spp. in 17 seafoods samples by qPCR and v-qPCR and comparison of results obtained by culture isolation.
| Log number of copies/g of flesh and intervalval liquid of shellfish |
Culture |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PE + NaCl | PE + NaCl | |||||||||
| Sample | D qPCR | D v-qPCR | PE qPCR | PE v-qPCR | qPCR | v-qPCR | D-BAa | D-MAb | PE-BAc | PE-MAd |
| Mussel 1 | 4.23 | ND | 5.20 | 5.19 | 5.63 | 5.36 | – | – | – | – |
| Mussel 2 | 4.72 | 4.13 | 4.22 | ND | 8.45 | 6.78 | – | – | – | – |
| Mussel 3 | 4.43 | 3.97 | 6.65 | 3.45 | 4.26 | 4.28 | – | – | – | – |
| Mussel 4 | 4.61 | 3.84 | 4.06 | 3.41 | 8.31 | 7.55 | – | – | – | – |
| Mussel 5 | 5.14 | 3.69 | 5.03 | 4.02 | 4.75 | 3.91 | – | – | – | – |
| Oyster 1 | 3.67 | 3.60 | 5.34 | 5.01 | 5.55 | 5.18 | – | – | – | – |
| Oyster 2 | 4.00 | 3.95 | 5.63 | 5.48 | 5.11 | 4.94 | – | – | – | – |
| Razor clam 1 | 4.84 | 4.21 | ND | ND | 5.79 | ND | – | – | – | – |
| Razor clam 2 | 4.32 | 3.63 | 4.58 | 4.81 | 4.84 | 4.41 | – | – | – | + |
| Razor clam 3 | 3.70 | ND | 5.74 | 5.97 | 6.27 | 5.57 | – | – | + | – |
| Wedge clam 1 | 6.71 | 6.70 | 6.35 | 6.32 | 5.54 | 5.32 | + | – | – | + |
| Wedge clam 2 | 6.73 | 6.39 | 8.73 | 8.70 | 6.07 | 5.86 | – | – | + | – |
| Wedge clam 3 | 4.91 | 4.81 | 5.09 | 3.80 | 7.78 | 5.74 | – | – | + | + |
| Wedge clam 4 | 3.92 | 4.13 | 4.85 | 4.66 | 4.46 | 4.34 | – | – | – | – |
| Wedge clam 5 | 5.06 | 4.01 | 5.13 | 5.00 | 4.84 | 4.21 | – | – | + | + |
| Wedge clam 6 | 4.13 | 4.19 | 5.32 | 4.08 | 6.22 | 4.12 | – | – | – | + |
| Wedge clam 7 | 4.01 | 4.09 | 5.02 | 5.19 | 6.13 | 5.23 | – | – | + | + |
| Average | 4.65 | 3.84 | 5.11 | 4.42 | 5.88 | 4.87 | ||||
D, direct samples; PE, post-enrichment in Arcobacter-CAT broth; PE + NaCl, post-enrichment in Arcobacter-CAT broth supplemented with 2.5% NaCl. aD-BA, homogenized samples directly cultured onto blood agar and incubated at 30°C for 48 h. bD-MA, homogenized samples directly cultured onto marine agar and incubated at 30°C for 48 h. cPE-BA, post-enrichment in Arcobacter-CAT broth cultured onto blood agar and incubated at 30°C for 48 h. dPE-MA, post-enrichment in Arcobacter-CAT broth supplemented with 2.5% NaCl cultured onto marine agar and incubated at 30°C for 48 h.