Figure 4.

TNFα production from mouse RAW 264.7 cells in response to stimulation with synthetic αTDE compounds. The indicated compounds were dissolved in 50% isopropanol/isooctane, serially diluted in vehicle and then dried to the bottom of a tissue culture plate. RAW 264.7 cells were applied to the compound-coated plates and incubated at 37°C; supernatant was harvested 24 h later and analyzed for TNFα via ELISA (A-E). Results from the 200 μg/ml concentration vs. each compounds acyl chain length are shown in (F). Data points are average of at least three independent experiments, for ease of interpretation ± SEM are only displayed in (F).