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Summary of purification procedure of ASNase.
| Purification Steps | Enzyme activity (U) | Protein (mg) | Specific activity (U/mg) | Fold Purification | Yield (%) |
|---|---|---|---|---|---|
| Crude extract | 890.0 | 114.4 | 7.78 | 1.00 | 100 |
| Ammonium sulfate and dialysis | 790.5 | 83.55 | 9.46 | 1.21 | 88.82 |
| Gel filtration chromatography | 415.0 | 11.50 | 36.08 | 4.63 | 46.62 |
