Figure 1.

PARP1 depletion destabilizes the mRNA of AKAP200 and CAPER. Half-lives of AKAP200 and CAPER were measured using qRT-PCR in WT and PARP1-KD cells. S2 Drosophila cells were transfected with siCon and siPARP1. The decay rates of total mRNAs were assessed using quantitative RT-PCR in WT and PARP1 KD cells following transcription inhibition by α-amanitin experiments (A,B) and DRB (C,D). Mean mRNA half-lives were calculated in WT and PARP1 KD for AKAP and CAPER transcripts previously shown to be bound by PARP1 at exon/intron boundaries and also alternatively spliced in PARP1 KD cells. Data are represented as mean of three experimental replicates ± SEM. Differences between WT and each experimental condition were measured and results were significant as measured by student T-test, p < 0.05.