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. 2019 Mar 6;9:3722. doi: 10.1038/s41598-019-39969-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Depletion of PARP1/PARylation affects transcription and PolII recovery rate as measured by nascent mRNA levels. Time-course of transcription elongation for AKAP and CAPER genes post-DRB treatment. WT and PARP1 KD cells were incubated with DRB for 3 hrs to halt transcription. Then post-DRB wash, transcription was allowed to start and continue in a synchronized manner. Levels of pre-mRNA as measured by RT-PCR using exon-intron junction primers (green arrows) were determined for AKAP200 (A) and CAPER (B) and PARP1 KD cells for AKAP200 (C) and CAPER (D). Pre-mRNA levels were normalized to the values prior of the prior-DRB treatment sample, which was set to 1. Results are shown as mean ± SEM from three independent experiments. Differences between NT and each experimental condition were considered significant according to student T-test p < 0.05.