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. 2019 Mar 6;9:3722. doi: 10.1038/s41598-019-39969-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PARP1-mediated coupling of RNAPII elongation and splicing kinetics is impaired in PARPI depletion. PCR products with exon junction spanning primers validate splicing changes in AKAP200 (A) and CAPER (B). Agarose gel images show the difference in splicing products between wildtype-non-treated (WT) and PARP1 knockdown (KD) cells before DRB treatment and at 90 min post-DRB recovery time point (WT90 and KD90 respectively). Actin was used as a PARP1-non-target control. The intensity of all bands per lane was quantified and the total band quantification was set to 100%. The relative amount of each band per lane was calculated as a percentage of the total band intensities per lane. The log values of the relative amount of each band to the total PCR product per lane for AKAP (C) and CAPER (D). Log values were plotted for easier visualization of the differences.