Figure 3.

Pyruvate-depletion induced dispersion is dependent on MifR and functional ldhA. (A,B) Biofilms by indicated P. aeruginosa PAO1 mutant strains grown for 4 days in 24-well polystyrene plates that were left untreated or exposure to 10 mU PDH and cofactors for 16 h. (A) Representative confocal images and (B) quantitative analysis of the biofilm biomass by P. aeruginosa PAO1 and indicated mutant strains. (C) Remaining biofilm biomass of P. aeruginosa PA14 and indicated mutant strains that were either left untreated or exposure to 10 mU PDH and cofactors for 16 h, as determined using CV staining. White size bar = 100 µm. *Statistically different from untreated biofilms (p < 0.05). All experiments were carried out at least in triplicate. Error bars denote standard deviation.