Table 1.
Strains and plasmids.
| Strains/plasmids | Relevant genotype or description | Source |
|---|---|---|
| Strains | ||
| Pseudomonas aeruginosa | ||
| PAO1 | Wild type | B.H. Holloway |
| ∆ldhA | PAO1; PA0927::ISlacZ; TetR | 81 |
| ∆mifR | PAO1, ∆mifR (PA5511) | 45 |
| ∆bdlA | PAO1, ∆bdlA | 47 |
| ∆dipA | PAO1, ∆dipA (PA5017) | 46 |
| ∆rbdA | PAO1, ∆rbdA | 46 |
| PA14 | Wild type | 82 |
| ∆mifR::IS | PA14 mifR::MAR2xT7; GmR | 83 |
| ∆ldhA::IS | PA14 ldhA::MAR2xT7; GmR | 83 |
| Pseudomonas aeruginosa clinical isolates | ||
| Chronic wounds | PA215; P. aeruginosa isolated from a chronic wound debridement samples from patients at Southwest Regional Wound Clinic (Lubbuk, TX) | 84, 85 |
| Cystic fibrosis | CF1–2; Classic P. aeruginosa isolate from newborn diagnosed with CF | 86 |
| ATCC 27312 | Isolated from infected human wound infection; used for second-degree burn wound studies | ATCC |
| Staphylococcus aureus | ||
| ATCC 6538 | Wild type | ATCC |
| Plasmids | ||
| pRK2013 | Helper plasmid for triparental mating; mob; tra; KmR | 87 |
| pJN105 | Arabinose-inducible gene expression vector; pBRR-1 MCS; araC-PBAD; GmR | 88 |
| pJN-mifR | mifR, amplified using PA5511_NheI_for/PA5511_EcoRI_rev, cloned int amplified using ldhA_NheI_for/ldhA_SacI_rev, cloned into pJN105, GmR | 45 |
| pMJT-1 | Arabinose-inducible gene expression vector; pUCP18 MCS; araC-PBAD; Amp/CarbR | 89 |
| pMJT-ldhA | ldhA, amplified using ldhA_NheI_for/ldhA_SacI_rev, cloned into pMJT-1, Amp/CarbR | 45 |