Fig. 1.
Defective humoral response in Ufbp1F/FCD19cre mice. a Flow cytometric detection of B220- and CD19-positive cells in spleens of indicated mice. Numbers represent the percent positive cells in the corresponding quadrant. b Presence of indicated immunoglobulin isotypes in the serum of indicated mice was quantified using enzyme-linked immunosorbent assay (ELISA) (n = 4 mice/genotype). c Six-week-old Ufbp1F/F and Ufbp1F/FCD19cre mice were immunized with 4-hydroxy-3-nitrophenylacetyl conjugated to keyhole limpet hemocyanin (NP-KLH; 100 µg/mouse, intraperitoneally) in alum. NP-specific antibodies of IgM, IgG1, IgG2a, IgG2b, and IgG3 isotypes in the sera of the mice at indicated time points after immunization were quantified using ELISA (n = 5 mice/genotype). Samples were normalized against day 14 sera obtained from wild-type mice immunized with NP-KLH. d Ufbp1F/F and Ufbp1F/FCD19cre mice were immunized with NP-Ficoll (25 µg/mouse) intraperitoneally in phosphate-buffered saline. NP-specific antibody of IgG3 isotype in the sera of mice at indicated time points after immunization was quantified as above (n = 3 mice/genotype). Error bars represent mean ± standard error. *P < 0.05, **P < 0.01, ***P < 0.001. Unpaired Student’s two-tailed t-test was used. A representative of at least two experiments is shown