Fig. 5.

An essential role of Ufbp1 in regulation of unfolded protein response signaling. a Flow cytometric detection of spliced XBP1 (XBP1s) expression in bone marrow plasma cells of mice of indicted genotype. Dotted and solid lines represent the staining for control isotype and anti-XBP1s antibodies respectively. b Quantification of XBP1s expression in a (n = 3 mice/genotype). c, f Naive B cells from Ufbp1^F/F and Ufbp1^F/FCD19^cre mice were stimulated with lipopolysaccharide. At indicated time points, cells were harvested and total cell lysates were immunoblotted with antibodies against indicated molecules. d, g Quantification of expression of indicated molecules normalized against β-actin in c and f respectively. e, h Data on indicated days from d and g were replotted. Expression value of indicated molecules in cells from Ufbp1^F/F mice on indicated time point was taken as 1. Error bars represent mean ± standard error. **P < 0.01. ns nonsignificant. Unpaired Student’s two-tailed t-test was used. A representative of at least two experiments is shown