Figure 2.

Purification and biophysical characterization of TEV-BM2(1–51). (a) SDS-PAGE gel of purified BM2, which runs as a mixture of monomers to tetramers under denaturing conditions. The molecular weight of the whole construct is 9.8 kDa. (b) CD spectra of BM2 in 0.5% DPC micelles and POPC: POPG bilayers at pH 7.5. Both spectra exhibit two minima at 209 nm and 222 nm that are characteristic of the α-helical conformation.