Figure 3.

1D ¹³C and ¹⁵N NMR spectra of BM2 in DLPE and POPC: POPG (4:1) bilayers at different temperatures. (a) ¹³C CP spectra, which selectively detect immobilized residues. (b) ¹³C DP spectra, which detect all residues’ signals. (c) ¹³C INEPT spectra, which selectively detect the signals of highly dynamic residues. (d) ¹⁵N CP spectra of immobilized residues. Top row shows the DLPE spectra measured at 285 K. Middle row shows the POPC: POPG spectra measured at 290 K. Bottom row shows the 260 K spectra of the POPC: POPG sample. Signals from the N-terminal solubility tag are predominantly seen in the INEPT spectra, whereas the TM signals dominate the CP spectra.