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. 2019 Mar 6;9:3725. doi: 10.1038/s41598-019-40217-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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2D ¹³C-¹³C correlation spectra of DLPE-bound BM2. (a) Aliphatic and carbonyl regions of the 2D spectrum measured with 55 ms ¹³C spin diffusion. The ¹³C linewidth is 0.8–1.1 ppm. (b) Aliphatic-aromatic regions of the 2D spectra measured with 100 ms (left) and 300 ms (right) ¹³C spin diffusion. The functionally important H19 and W23 in the HxxxW motif and the C-terminal H27 are assigned.