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. 2019 Mar 6;9:3662. doi: 10.1038/s41598-019-40094-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased FAM83H-AS1 expression in primary cervical keratinocytes containing the HPV-16 genome as well as in HPV-16 positive cervical cancer and head and neck squamous cell carcinoma cell lines. (A) qRT-PCR analysis showing the increase of FAM83H-AS1 expression in newly immortalized cervical keratinocytes expressing entire HPV-16 genome (JAMM-16) compared to uninfected primary cervical keratinocytes (HCK). (B) qRT-PCR analysis showing the increase of FAM83H-AS1 expression in HPV-16 positive cervical cell lines (CaSki, W12/20863, W12/201402) and decrease of FAM83H-AS1 in HPV negative cervical cancer cell line (C-33A) compared to uninfected cervical keratinocytes (HCK). (C) qRT-PCR analysis showing the increase of FAM83H-AS1 expression in HPV-16 positive head and neck squamous cell carcinoma (HNSCC) cell lines (UMSCC-47 and UMSCC-104) compared to HPV negative HNSCC cell line (UMSCC-1). All graphs in the figure show the average of two individual experiments. Similar results were obtained in at least three independent experiments. GAPDH mRNA was used to normalize the qRT-PCR analyses. Two-tailed t test results are indicted as **p ≤ 0.01.