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. 2019 Mar 6;9:3662. doi: 10.1038/s41598-019-40094-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Regulation of FAM83H-AS1 expression by HPV-16 E6 in a p53-independent, p300-dependent manner. (A) FAM83H-AS1 expression by qRT-PCR analysis in cervical keratinocytes (HCK) stably individually expressing HPV-16 E6 or E7, or co-expressing E6/E7 compared to GFP control. (B–C) qRT-PCR analysis of HPV-16 E6 and FAM83H-AS1 expression in HPV-16 positive CaSki (B) and W12/201402 (C) cervical cell lines transfected with an siRNA against HPV-16E6 compared to siRNA control. (D) qRT-PCR analysis of p53 and FAM83H-AS1 expression in HCK transfected with an siRNA against p53 compared to siRNA control. (E) Genome representative image showing location of FAM83H, FAM83H-AS1, and three predictive p300 binding sites in FAM83H-AS1 promoter region. (F) p300 and FAM83H-AS1 expression in HCK transfected with an siRNA against p300 compared to siRNA control. All graphs in the figure show the average of two individual experiments. Similar results were obtained in at least three independent experiments. GAPDH mRNA was used to normalize the qRT-PCR analyses. Two-tailed t test results are indicted as *p ≤ 0.05 and **p ≤ 0.01. CTRL, control.