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. 2019 Mar 6;9:3662. doi: 10.1038/s41598-019-40094-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FAM83H-AS1 is localized in the nucleus in cervical pre-malignant and cancerous cell lines. (A) qRT-PCR of FAM83H-AS1 expression in fractionated HPV-16 positive cervical cancer CaSki (A) and pre-malignant W12/201402 (B) cell lines. U6 small nuclear RNA (snRNA) was used as a nuclear control RNA and mature Beta Actin was used as a cytoplasmic control RNA. Representative images; similar results were obtained in at least three independent experiments. Normalization was done using C. Elegans total RNA as an exogenous spike for the amplification of worm-specific ama-1 gene. Two-tailed t test results are indicted as **p ≤ 0.01.