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. 2019 Mar 6;10:1085. doi: 10.1038/s41467-019-08977-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Rapid plasticity alters Unc13A/BRP/RBP AZ protein cluster number. a STED microscopy images containing several AZs with variable numbers of protein clusters of Unc13A (magenta), BRP (green) and RBP (red). Dashed white boxes mark one AZ example used for the cluster number counting. b Example AZs with 2–6 Unc13A clusters, marked by colored dots and used for cluster number counting. (c–e, left) Frequency distribution of Unc13A (c), BRP (d), and RBP (e) modules per AZ either without (Ctrl, −PhTx; black) or with PhTx (+PhTx; blue) treatment. f–h Average of rotated STED images stained against Unc13A (f), BRP (g), and RBP (h) with 2–6 modules either without (Ctrl) or with PhTx (+PhTx) treatment. See also Supplementary Figs. 2–4. Source data as exact values, detailed statistics including sample sizes and P values are provided in the Source Data file. Scale bars: a 200 nm; (b, f–h) 50 nm. Statistics: Mann-Whitney U test. *P ≤ 0.05; **P ≤ 0.01. Panels (c–e) show NMJ-wise means of AZ-mean ± s.e.m.