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. 2019 Mar 6;10:1085. doi: 10.1038/s41467-019-08977-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Rapid AZ-remodeling during PHP requires Aplip-1 and Srpk79D. a, b Muscle 4 NMJs of segment A2-A5 from third instar larvae and quantification of synaptic levels of wild-type—1, aplip-1^Null, aplip-1^ek4, wild-type-2, atg1 and srpk79D^ATC labelled with the indicated antibodies without (Ctrl; black) and with 10 min of PhTx (+PhTx; blue) treatment. Two independent experiments were performed, wild-type-1 was used as control for aplip-1^Null and aplip-1^ek4 while wild-type-2 was used for atg1^Null and srpk79D^ATC. c Average BRP and Unc13A cluster number per AZ either without (Ctrl, −PhTx; black) or with PhTx (+PhTx; blue) treatment in aplip-1^ek4 and srpk79D^ATC. See also Supplementary Figs. 5, 6 and Movie 1. Source data as exact normalized and raw values, detailed statistics including sample sizes and P values are provided in the Source Data file. Scale bars: a, b 5 µm; c 500 nm. Statistics: Mann–Whitney U test. * P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; n.s., not significant, P > 0.05. All panels show mean ± s.e.m.