Fig. 4.

Rapid AZ-remodeling during PHP requires BRP and RBP. a, b Confocal images and quantification of synaptic intensities of muscle 4 NMJs of abdominal segment 2–4 from third instar larvae at wild-type, rbp^Null, brp^Null, brp^Null/+, rim^Null, rim^Null/fife^Null, liprin-α^Null, and syd1^Null NMJs labelled with the indicated antibodies without (control; black) and with 10 min PhTx (+PhTx; blue) treatment. For wild-type, images and data were adapted and replotted from Fig. 1. Please note, although wild-type controls were performed in parallel to every mutant genotype to check for functional AZ-remodeling upon PhTx-treatment in each set of experiments, we do not show all WT-control here due to space limitations. Therefore, AZ-protein levels should not be compared between genotypes. Source data as exact normalized and raw values (also of additional Wild-type controls for genotypes where PhTx-treatment failed to induce AZ-remodeling), detailed statistics including sample sizes and P values are provided in the Source Data file. Scale bars: 5 µm. Statistics: Mann–Whitney U test. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; n.s., not significant, P > 0.05. All panels show mean ± s.e.m.