Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 6;10:1085. doi: 10.1038/s41467-019-08977-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — AZ-remodeling sustains NT-release potentiation over longer timescales. a Sketch of investigated conditions for rapid plasticity: control synapses (left) are compared with rapid plasticity (10 min of PhTx (red “X”); right). Rapid plasticity increases the number of SVs released (red). b, c (left) Representative traces of eEPSP (evoked) and mEPSP (spont.) of the indicated genotypes with and without PhTx-treatment. (Right) Quantifications of percentage change of mEPSP amplitude, eEPSP amplitude and quantal content (QC) upon PhTx-treatment. Values are divided by the corresponding measurement in the absence of PhTx for each genotype (dashed red line corresponds to 100%/no change). d (left) Confocal images of muscle 4 NMJs of abdominal segment 2–5 from third instar larvae at wild-type and srpk79D^ATC NMJs labelled with the indicated antibodies without (control; black) and with 10 min PhTx (+PhTx; blue) treatment. (Right) Quantification of percentage change of synaptic BRP and Unc13A levels in wild-type (blue) and srpk79D^ATC (orange) upon PhTx-treatment compared to baseline of control treatment for each genotype (dashed red line). Data are modified from Fig. 2. e Sketch of investigated conditions for chronic plasticity: wild-type synapses (left) are compared with gluRIIA^Null mutants. Chronic plasticity greatly increases the number of SVs released. f, g Same as in (b, c) but compared to baseline of each control genotype. h Same as in (d) but compared to baseline fluorescence values of wild-type for gluRIIA^Null and srpk79D^ATC for gluRIIA^Null;srpk79D^ATC. See also Supplementary Fig. 7. Source data as exact normalized and raw values, detailed statistics including sample sizes and P values are provided in the Source Data file. See also Supplementary Figure 10 and 11 for non-normalized values. Scale bars: eEPSP: 25 ms, 5 mV; mEPSP: 50 ms, 1 mV; d, h 5 µm. Statistics: Student’s unpaired T-test was used for comparisons in ((b) quantal content change), (f), (g) and Mann–Whitney U test for all other comparisons. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; n.s., not significant, P > 0.05. All panels show mean ± s.e.m.