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. 2019 Mar 6;9:3755. doi: 10.1038/s41598-019-40255-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Clinical grade and pixel coverage value of conjunctival hyperemia. Typical photograph of each clinical grade is shown (A). The software calculates the proportion of the conjunctival blood vessels as the pixel coverage. The pixel coverage value was automatically calculated for the closed square area (vertical 60% and horizontal 40%). When processing the photographs shown in (A) to the analysis software, blood vessels are drawn in the green area (B).