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. 2019 Mar 6;10:1089. doi: 10.1038/s41467-019-09052-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Abilities of mutant ES cells lacking Nodal/Bmp/Smad pathway components to undergo PGCLC differentiation. a Time-line and culture protocol used for PGCLC differentiation. b Stella IF staining of day 2 and day 4 BV⁺ PGCLC aggregates derived from the indicated genotypes, counter stained with DAPI. Scale bars = 100 μm. c Heatmap showing the log2 fold changes (log2FC) of Prdm1, Tfap2c and Hoxb1 expression, as judged by RT-qPCR, at day 2, 4 and 6 of PGCLC differentiation in control and mutant cells of the indicated genotypes, relative to EpiLCs