Fig. 3.

DC-STAMP p.L397F variant attenuates NFκB and AP-1 activity. HEK293 cells stably expressing pEGFP N3 Empty Vector (EV), or pEGFP N3 DC-STAMP WT or p.L397F were co-transfected with a Renilla internal control expression plasmid and either (A) an NFκB luciferase reporter plasmid or (B) a AP-1 luciferase reporter plasmid. After 48 h, luciferase activities were read and firefly luciferase values were first normalised to Renilla, and then compared directly to the wildtype level set at 100%. (C) Expression levels of pEGFP N3 DC-STAMP WT or p.L397F were determined by western blot against GFP with total protein used as a loading control. Individual assays were performed in quadruplicate, and data is the average relative luciferase units (RLU) of 3 independent experiments ± Standard Error. p values indicate significant differences compared to cells expressing wild type DC-STAMP.