FIG 1.

(A) Map of a circular plasmid showing relative position of selection markers (CaURA3, CaHIS1, CdARG4, and CmLEU2) and ORI sequence. (B) pCir-CaURA3 plasmid with different origin sequences transformed in C. albicans BWP17: transformation efficiency, types of colonies, and their mitotic stability. The transformation efficiency is an average from three independent experiments. (C) Comparison of circular plasmids carrying different selection markers with and without ORI410: transformation efficiency, proportion of different types of transformants, and log₂ value of the ratio of average number of transient or autonomous transformants with ORI to that without ORI (ORI410/No ORI). CaURA3, CaHIS1, CdARG4, and CmLEU2 plasmids were transformed in C. albicans BWP17, SN76, SN76, and SN152, respectively. Different markers are represented by different colors, and different types of transformants are represented by various shades of a color (lightest shade representing transients, intermediate shade representing ARS-transformants, and darkest shade representing integrants). The transformation efficiency is an average from three independent experiments. The size of circles showing proportion of transformant types is an approximation of transformation efficiency. (D) Mitotic stability (%) of integrants and ARS-transformants and plasmid loss rate/generation for ARS-transformants obtained with different circular plasmids with and without ORI410. The data represent the average from three independent colonies of each type. Int, integrants; ARS, ARS-transformants.