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. 2019 Feb 7;17(4):3874–3880. doi: 10.3892/ol.2019.10023

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Copyright: © Wen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Downregulation of LINC00460 inhibits the proliferation and migration of bladder cancer cells. (A) The green fluorescent protein images indicate the efficiency of transfection. Magnification, ×100. (B) Reverse transcription quantitative polymerase chain reaction assays demonstrated that LINC00460 was significantly downregulated following short hairpin RNA transfection. (C) The downregulation of LINC00460 inhibited the proliferation of bladder cancer cells in vitro. The knockdown of LINC00460 decreased the distance of migration in bladder cancer (D) 5637 and (E) T24 cells. Magnification, ×40. The experiments were performed in triplicate and data are expressed as the mean ± standard deviation. *P<0.05. OD, optical density; NC, negative control; RNAi, RNA interference; sh, short hairpin.