Microscopic results presenting the expression of NY-ESO-1 and MAGE-A4 in synovial sarcoma (magnification, ×40). (A and B) The clone E978 recognizes the NY-ESO-1 protein. (C and D) MCV-1 recognizes the MAGE-A2, MAGE-A4, and MAGE-A12 protein, and (E and F) MCV-4 recognizes the MAGE-A1 and MAGE-A4 protein. A case of positive staining with clone (A) E978, (C) MCV-1 and (E) MCV-4, respectively. A case of negative staining with clone (B) E978, (D) MCV-1 and (F) MCV-4, respectively. NY-ESO-1, New York-esophageal squamous cell carcinoma-1; MAGE-A4, melanoma-associated antigen-4; MCV, Mie Cancer Vaccine.