Skip to main content
. 2018 Jul 19;10(7):791. doi: 10.3390/polym10070791

Figure 3.

Figure 3

Normalized frequency, F/n, (blue lines) and dissipation, D, (red lines) shifts for gold-coated quartz crystal upon the adsorption of the P(NIPAM-co-APMA) microgel (a,c) or the P(NIPAM-co-DMAPMA) microgel (b,d), followed by the adsorption of ChO. Conditions: (Step 1) microgel adsorption from 1 g/L at pH 9.3 at 25 °C (a,b) or 50 °C (c,d); (Step 1′) temperature-induced swelling of the microgel film at pH 9.3 upon a temperature decrease from 50 to 25 °C; (Step 1″) temperature-induced deswelling of the microgel film at pH 9.3 upon a temperature increase from 25 to 50 °C; (Step 2) ChO uptake from the solution with the enzyme concentration of 0.4 g/L at pH 7.0 by the microgel film at 25 °C, in the case of the microgel film deposited at 50 °C, the enzyme uptake takes place simultaneously with microgel swelling upon a temperature jump from 50 to 25 °C (‘spongelike’ adsorption).