Figure 7.

Dissociation rates of S₆-containing peptides from the α-HL depend on the polarity of the applied ΔV and the side on which they are added (cis or trans). All the graphs display the voltage-dependent dissociation rate constants (k_off) of the Pe6 peptide [30 μM] from the α-HL. As in Figure 5: (a,b) the peptides were added to the trans side; and (c,d) the peptides were added on the cis side. The dotted lines represent the upper and lower limits of the 95% confidence intervals for the estimated average values [40].