Skip to main content
. 2018 Jun 21;10(7):690. doi: 10.3390/polym10070690

Figure 5.

Figure 5

(a) Cell viability of HMFCs on peptide hydrogels. HMFCs were cultured on hydrogels containing peptides Fmoc-FRGDF, Fmoc-FRGDFPHSRN and peptide mixture (coassembled Fmoc-FRGDF/Fmoc-PHSRN) for three days. MTS assay was performed and the absorbance values were obtained at a wavelength of 490 nm. Absorbance value of cells grown on Fmoc-FRGDF was considered as control. A p-value of <0.05 was considered significant. (b) Analysis of spreading area: HMFCs were cultured on hydrogels containing peptides for 48 h. Cells were stained with actin and the images were obtained using fluorescent microscope (20× magnification). ImageJ analysis was performed and the values were plotted in a graph using GraphPad Prism, the p-value **** <0.05 was considered statistically significant. (c) Actin staining of HMFCs growing on hydrogels shows typical morphologies for each condition measured in (b) scale bar 10 µm.