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. 2018 Sep 25;10(10):1061. doi: 10.3390/polym10101061

Figure 5.

Figure 5

Phase contrast microscopy images of the morphology and differentiation of MC3T3-E1 cells. (A): cell morphology of MC3T3-E1cells; (B): Calcein-AM staining of the seventh generation of cells; (C): Hematoxylin-eosin (HE) staining of the seventh generation of cells; (D): Alkaline phosphatase staining after one week of osteogenic induction; (E): Alizarin red S staining after three weeks of osteogenic induction; (F): Von-Kossa staining after four weeks of osteogenic induction. Scale: 250 μm.