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(A) The time-course of adhesion and spread of MC3T3-E1 cells of P7 passage on the surface of the three microcarriers with the inoculation density of 1 × 105 cells/mL containing the stages of contact, adhesion, spreading, and proliferation; (B) The detachment process of MC3T3-E1 from microcarriers and live/dead staining of the detached cells which were reseeded into 24-well plates and cultured for one week. Scale: 250 μm.
