Skip to main content
. 2018 Sep 25;10(10):1061. doi: 10.3390/polym10101061

Figure 9.

Figure 9

(A): Adhesion, spread, and proliferation of MC3T3-E1 cells cultured on the surface of microcarriers for 24 h, 72 h, and 120 h observed by scanning electron microscopy; (B): a–c: Alkaline phosphatase staining of MC3T3-E1 cells inoculated onto the surface of microcarriers after one-week osteogenic induction (the green arrows indicated the cells-microcarriers complex in the 24-well plate stained by alkaline phosphatase). d–f: Alkaline phosphatase staining of MC3T3-E1 cells firstly inoculated into the 24-well plate and cultured for 3 days, then adhered to the microcarriers. Scale: 250 μm; (C): The adhesion determination of MC3T3-E1 cells on the surface of microcarriers; (D): The proliferation detection of MC3T3-E1 cells on the surface of microcarriers (n = 3, * P < 0.05); (E): ALP activity assay of MC3T3-E1 cells on the three kinds of microcarriers (n = 3, * p < 0.05, ** p < 0.01).