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. 2019 Mar 6;11:2155179019834938. doi: 10.1177/2155179019834938

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Cardiomyocyte exposure to 24 hours of 1% oxygen mimics the cellular phenotype of hibernating myocardium (HM). Measurement of cellular hypoxia at (a) baseline and (b) 24 hours following exposure to hypoxic conditions. (c) Quantification of hypoxia by measurement of fluorescent intensity (n=4; p=0.0076; nd=not detectable). (d) Cell viability was unchanged following exposure to 24 hours of hypoxia (n=4). (e) Measurement of oxygen consumption rate (OCR) indicated impaired respiratory capacity following hypoxia that was not restored following reoxygenation. Basal (f) and maximal (g) respiration was significantly reduced following hypoxia and was not restored following reoxygenation (p=0.0005 and p<0.0001, respectively).