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A
Qualitative analysis of lung structure in Richardson‐stained plastic‐embedded lung sections from wild‐type (WT) and miR‐34a−/− mice during normal and aberrant alveolarization (scale bar, 50 μm).
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B
Quantification of total number of alveoli by design‐based stereology in wild‐type (WT) and miR‐34a−/− mice (34a−/−) during normal and aberrant alveolarization.
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C
Quantification of mean septal thickness by design‐based stereology in wild‐type (WT) and miR‐34a−/− mice (34a−/−) during normal and aberrant alveolarization.
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D
Qualitative analysis of lung structure in Richardson‐stained plastic‐embedded lung sections from wild‐type (WT) and miR‐34bc−/− mice during normal and aberrant alveolarization (scale bar, 50 μm).
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E
Quantification of total number of alveoli by design‐based stereology in wild‐type (WT) and miR‐34bc−/− mice (34bc−/−) during normal and aberrant alveolarization.
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F
Quantification of mean septal thickness by design‐based stereology in wild‐type (WT) and miR‐34bc−/− mice (34bc−/−) during normal and aberrant alveolarization.
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G
Localization of miR‐34a expression by β‐galactosidase activity staining in the developing lungs of P14 miR‐34a::
lacZ
+/+ mice that were undergoing normal or aberrant alveolarization (scale bar, 50 μm; larger and some additional images are presented in
Appendix Fig S2).
Data information: Qualitative data (A, D, G) illustrated from one experiment are representative of the trend observed in four other (A, D) or two other (G) experiments. For all quantitative data sets (B, C, E, F), five animals for each experimental group are illustrated, with each data point representing an individual animal, where data represent mean ± SD.
P values for selected comparisons were determined by one‐way ANOVA with Tukey's
post hoc modification.
