. 2018 Nov 13;8(2):188–195. doi: 10.1039/c8tx00233a

Table 1. Flow cytometric cell cycle analysis in HepG2 cells incubated with chromium chloride, iron chloride, nickel chloride, and molybdenum trioxide.

		BALB/3T3 clone A31 cells				HepG2 cells
		Cell number [%]				Cell number [%]
		subG1	G0/G1	S	G2/M	subG1	G0/G1	S	G2/M
Concentration of chromium chloride [μM]
0		6 ± 0.5	58 ± 4	9 ± 0.5	27 ± 2	7 ± 0.5	59 ± 4	9 ± 0.5	25 ± 1
200		7 ± 0.4*	55 ± 3*	9 ± 0.6	29 ± 1*	9 ± 0.7*	54 ± 5*	8 ± 0.3	29 ± 2*
400		10 ± 1*	47 ± 3*	9 ± 0.4	34 ± 3*	11 ± 1*	45 ± 3*	8 ± 0.4	36 ± 2*
600		14 ± 1*	33 ± 3*	9 ± 0.7	44 ± 3*	15 ± 1*	30 ± 1*	7 ± 0.5*	48 ± 1*
800		15 ± 1.2*	25 ± 1*	5 ± 0.2*	55 ± 4*	17 ± 1*	24 ± 2*	3 ± 0.1*	56 ± 4*
1000		16 ± 1*	17 ± 1*	2 ± 0.1*	65 ± 5*	19 ± 1*	14 ± 1*	1 ± 0.1*	66 ± 4*
Concentration of iron chloride [μM]
0		6 ± 0.5	58 ± 4	9 ± 0.5	27 ± 2	7 ± 0.5	59 ± 4	9 ± 0.5	25 ± 1
200		7 ± 0.3	60 ± 3*	9 ± 0.2	24 ± 1*	8 ± 0.2	61 ± 3*	9 ± 0.2	22 ± 1*
400		8 ± 0.2*	62 ± 2*	9 ± 0.3	21 ± 1*	10 ± 1*	64 ± 4*	7 ± 0.3*	19 ± 1*
600		9 ± 0.5*	67 ± 5*	8 ± 0.2*	16 ± 1*	12 ± 1.1*	70 ± 5*	6 ± 0.1*	12 ± 0.8
800		11 ± 1*	70 ± 2*	7 ± 0.1*	12 ± 0.9*	14 ± 1*	74 ± 4*	4 ± 0.1*	8 ± 0.3*
1000		13 ± 1*	72 ± 3*	7 ± 0.4*	9 ± 0.3*	15 ± 0.8*	75 ± 3*	3 ± 0.1*	7 ± 0.2*
Concentration of molybdenum trioxide [μM]
0		6 ± 0.5	58 ± 4	9 ± 0.5	27 ± 2	7 ± 0.5	59 ± 4	9 ± 0.5	25 ± 1
200		7 ± 0.4	49 ± 3*	9 ± 0.4	35 ± 2*	8 ± 0.4	47 ± 3*	9 ± 0.6	36 ± 3*
400		8 ± 0.2*	45 ± 4*	8 ± 0.6	39 ± 3*	9 ± 0.6*	44 ± 3	7 ± 0.4*	40 ± 2*
600		10 ± 1*	40 ± 2*	7 ± 0.4*	43 ± 3*	10 ± 1*	38 ± 2*	5 ± 0.1*	47 ± 3*
800		12 ± 1*	32 ± 2*	5 ± 0.2*	51 ± 5*	13 ± 1*	31 ± 1*	4 ± 0.2*	52 ± 4*
1000		15 ± 1*	20 ± 1*	5 ± 0.3*	60 ± 4*	17 ± 1*	17 ± 1*	3 ± 0.1*	63 ± 4*
Concentration of nickel chloride [μM]
0		6 ± 0.5	58 ± 4	9 ± 0.5	27 ± 2	7 ± 0.5	59 ± 4	9 ± 0.5	25 ± 1
200		3 ± 0.2*	58 ± 3*	9 ± 0.7	30 ± 3*	3 ± 0.1*	57 ± 3	9 ± 0.8	31 ± 2*
400		5 ± 0.5*	54 ± 4*	8 ± 0.6	33 ± 3*	6 ± 0.5*	52 ± 5*	8 ± 0.5	34 ± 3*
600		6 ± 0.4*	50 ± 3*	7 ± 0.5*	37 ± 2*	8 ± 0.6*	46 ± 2*	6 ± 0.2*	40 ± 3*
800		11 ± 1*	44 ± 2*	5 ± 0.3*	40 ± 1*	9 ± 0.5*	37 ± 3*	4 ± 0.1*	50 ± 4*
1000		13 ± 1*	37 ± 2*	1 ± 0.1*	49 ± 4*	14 ± 1*	28 ± 1*	1 ± 0.1*	57 ± 5*
Concentration (mixture of metal compounds)
200 μM	1000 μM
CrCl₃·6H₂O	FeCl₃·6H₂O	21 ± 1*^,1,2	77 ± 6*^,1,2	1 ± 0.1*^,1,2	1 ± 0.1*^,1,2	22 ± 1*^,1,2	76 ± 6*^,1,2	1 ± 0.1*^,1,2	1 ± 0.1*^,1,2
CrCl₃·6H₂O	MoO₃	5 ± 0.2*^,1,2	55 ± 4*^,1,2	10 ± 1*^,1,2	30 ± 2*^,1,2	6 ± 0.2*^,1,2	54 ± 4*^,1,2	10 ± 1*^,1,2	30 ± 2*^,1,2
CrCl₃·6H₂O	NiCl₂·6H₂O	5 ± 0.1*^,1,2	50 ± 3*^,1,2	12 ± 1*^,1,2	33 ± 2*^,1,2	5 ± 0.1*^,1,2	55 ± 2*^,1,2	15 ± 1*^,1,2	25 ± 1*^,1,2
1000 μM	200 μM
CrCl₃·6H₂O	FeCl₃·6H₂O	8 ± 0.2*	50 ± 4*	7 ± 0.2*	35 ± 1*	8 ± 0.2*	40 ± 3*	7 ± 1*	45 ± 3*
CrCl₃·6H₂O	MoO₃	6 ± 0.2*	35 ± 2*	9 ± 0.1*	50 ± 1*	7 ± 0.2*	36 ± 2*	10 ± 1*	47 ± 2*
CrCl₃·6H₂O	NiCl₂·6H₂O	5 ± 0.1*	40 ± 1*	10 ± 1*	45 ± 2*	4 ± 0.2*	36 ± 1*	5 ± 0.1*	55 ± 3*