Table 1.
Specific Primer Sequences Used in Real‐Time PCR Analysis
| Gene | Accession No. | Product Size | Primer Sequence |
|---|---|---|---|
| Gata4 | NM_008092.3 | 134 | Forward: GCCTGCGATGTCTGAGTGAC |
| Reverse: CACTATGGGCACAGCAGCTC | |||
| Gata5 | NM_008093.2 | 167 | Forward: ACCCCACAACCTACCCAGCA |
| Reverse: GCCCTCACCAGGGAACTCCT | |||
| Nkx2.5 | NM_008700.2 | 162 | Forward: GACAGCGGCAGGACCAGACT |
| Reverse: CGTTGTAGCCATAGGCATTG | |||
| Tbx5 | NM_011537.3 | 103 | Forward: AGGAGCACAGTGAGGCACAA |
| Reverse: GGGCCAGAGACACCATTCTC | |||
| Notch1 | NM_008714.3 | 142 | Forward: CAGCTTGCACAACCAGACAGA |
| Reverse: TAACGGAGTACGGCCCATGT | |||
| Gch1 | NM_008102.3 | 117 | Forward: TCAAGAGCGCCTCACCAAAC |
| Reverse: TTCTGCACGCCTCGCATTAC | |||
| Dhfr | NM_010049.3 | 153 | Forward: GAATCAACCAGGCCACCTCA |
| Reverse: TTGATGCCTTTTTCCTCCTG | |||
| 28S | NR_003279.1 | 178 | Forward: GGGCCACTTTTGGTAAGCAG |
| Reverse: TTGATTCGGCAGGTGAGTTG |
PCR indicates polymerase chain reaction.