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. 2018 Oct 23;7(21):e009700. doi: 10.1161/JAHA.118.009700

Figure 4.

Figure 4

Sirt1 antisense (AS) long noncoding RNA (lncRNA) drives cardiomyocyte (CM) proliferation in adults. A, Real‐time quantitative PCR analyses of Sirt1 AS lncRNA levels in adeno‐associated virus 9–negative control (AAV9‐NC) or AAV9–Sirt1 AS lncRNA (SAS) injected adult mice hearts 28 days after injection (n=6 mice per group). B, Ratios of heart weight/body weight in neonatal mouse hearts injected with AAV9‐NC or AAV9‐SAS (n=5 mice per group). C, Wheat germ agglutinin (WGA) staining of heart sagittal sections for AAV9‐NC and AAV9‐SAS adult mice hearts and quantification of CM size (175 CMs from 5 mice in AAV9‐NC group, 175 CMs from 5 mice in AAV9‐SAS group). Bar=25 μm. D, Ki67 immunofluorescence staining in AAV9‐NC and AAV9‐SAS adult mice hearts and quantification of ki67‐positive CMs (2591 CMs from 5 mice in AAV9‐NC group, 2680 CMs from 5 mice in AAV9‐SAS group). Ki67‐positive CMs were indicated by arrows. Bar=20 μm. E, pH3 immunofluorescence staining in AAV9‐NC and AAV9‐SAS adult mice hearts and quantification of pH3‐positive CMs (5714 CMs from 5 mice in AAV9‐NC group, 5870 CMs from 5 mice in AAV9‐SAS group). pH3‐positive CMs were indicated by arrows. Bar=20 μm. F, Ki67 immunofluorescence staining in AAV9‐NC and AAV9‐SAS adult mice hearts 28 days after myocardial infarction (MI) and quantification of ki67‐positive CMs (2690 CMs from 5 mice in AAV9‐NC group, 2695 CMs from 5 mice in AAV9‐SAS group). Ki67‐positive CMs were indicated by arrows. Bar=20 μm. G, pH3 immunofluorescence staining in AAV9‐NC and AAV9‐SAS adult mice hearts 28 days after MI and quantification of pH3‐positive CMs (6778 CMs from 5 mice in AAV9‐NC group, 6858 CMs from 5 mice in AAV9‐SAS group). pH3‐positive CMs were indicated by arrows. Bar=20 μm. Statistical significance was calculated using 2‐tailed unpaired Student t test in A through G. Data represent mean±SEM. *P<0.05.