Effect of losartan and PD on coronary resistance and cardiac work in APC hearts. (a) Changes in coronary resistance were determined during 1 and 5 min after the onset of angiotensin II (10 nM) in Langendorff-perfused hearts. The values shown are means ± of 5–14 experiments per groups. *p<0.05 vs Control, #p<0.05 vs AII, +p<0.05 vs AII+PD. AII = angiotensin II, L = losartan, PD = PD 123,319. (b) Rate pressure product (RPP) was determined 5 minutes before global ischemia and at 60 min of reperfusion. RPP values at 90 min were not significantly different from those at 60 min. The values shown are means ± SEM of 5–16 experiments per group. *p<0.05 vs Control; #p<0.05 vs A; &p<0.05 vs A+L. A = APC; A+L = APC+losartan; A+PD = APC+PD; A+L+PD = APC+losartan+PD