Effect of beam-film parameters on crossover patterning in Arabidopsis. The effect of altering a single beam-film parameter—L (A–C), Smax (D–F), or T2Prob (G–I)—on crossover distribution (A, D, and G) and CoC (B and C, E and F, and H and I). Red vertical lines in (C, F, and I) represent IID = 0.1. Gray shading in CoC curves indicates 95% C.I. CoC, coefficient of co-incidence; IID, interinterval distance; Smax, maximum stress level per bivalent; T2Prob, probability that a non-CO designated precursor will form a class II CO.