Figure 7.

The timing of CENP-A expression is dispensable for its centromeric targeting. (A) Schematic diagram of the cnp1-gfp constructs under promoters that drive expression at different cell cycle stages. (B) Quantitative RT-PCR analysis of RNA levels of cnp1-gfp driven by different promoters. Graphic shows fold change compared to the WT cnp1 promoter. (C) Western blotting analysis of Cnp1-GFP protein levels expressed by different promoters. Tubulin was used as a loading control. Short and long exposures are shown for anti-GFP blotting. (D) Distribution patterns of Cnp1-GFP expressed from different promoters. Percentage of cells showing multifoci phenotype is indicated for each sample. Exp., exposure; WT, wild-type.