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. 2019 Mar 6;15:79. doi: 10.1186/s12917-019-1810-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Determination of cut-off value for PCV3 Cap ELISA. Cut-off values were evaluated by testing 48 negative control sera. Each value represented the mean absorbance at 450 nm obtained from three replicates of each serum sample tested. The cut-off value was defined as mean OD value of all tested negative control sera, plus three standard deviations. The cut-off value (0.541) was represented by dashed lines. Error bars indicated mean and SD