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. 2019 Mar 6;20:177. doi: 10.1186/s12864-019-5548-x

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — iGEAK requires 3 input files: a two-column gene annotation file (a), a gene expression matrix (b), and a multi-column sample-group metadata file (c). The data pre-processing steps to prepare input files are briefly explained as diagram (d). iGEAK does not directly process raw CEL files or FASTQ/BAM files (open arrows, d)